Prokaryotic Cells

1. Bacteriology

2. Phage Preparation and Purification

   1. Bacteriophage preparation

   2. Packaging extracts for lambda cloning

   3. Bacteriophage M13

3. Preparation of Competent Cells

   1. RbCl2 method

   2. Shawn’s CaCl2

   3. DAM strains

4. Transformation

   1. Optimizing DNA for transformation

   2. Transforming competent cells

   3. Transforming DH5 cells

5. Library Screening