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Morimoto Lab 

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    Rice Institute for Biomedical Research

    Department of Molecular Biosciences

    Northwestern University

     

    Eukaryotic Cells

    1. Cell Culture

      1. Introduction to cell culture

      2. Media preparation

      3. DMEM

      4. Dialysis of FBS

      5. Splitting

      6. Hemacytometer

      7. Coulter counter

      8. Trypan blue staining

      9. Freeze/thaw

      10. Freezing cell lines

      11. Thawing cells

      12. HeLa S3 cell culture

      13. Xenopus oocyte microinjection

      14. Preparation of extracts from human placenta

      15. Chicken blood injections

      16. Sacrifice of adult hen

      17. Bleeding eggs

      18. Chicken embryonic fibroblasts

    2. Cell Transfection

      1. CaPO4 method

      2. 3% CO2 method

      3. Ecogpt

      4. Trypsinization transfection

      5. Polyfect, Lipofectamine, and Effectene

      6. Cell sorting for transfected cells: MACs protocol

    3. Establishing Stable Cell Lines

      1. Calcium Chloride Transfection

      2. Lipofectamine Transfection (Gen’s Protocol)

    4. Radiolabeling

      1. 32P orthophosphate and 35S methionine/cysteine

      2. Metabolic labeling

    5. Cell Growth/Death Assays

      1. Cell Death ELISA

      2. MTT

      3. TUNEL

      4. Drug Assays

    6. Luciferase Assays

      1. Luminometer operation

      2. Single luciferase

      3. Dual luciferase

      4. 96-well plate format

    7. RNAi – mammalian cells

    8. Cell Imaging

      1. Intro to immunofluorescence

      2. Method

      3. FRET

    9. Transferrin Uptake using Fluorescence Microscopy

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