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Morimoto Lab 

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    Rice Institute for Biomedical Research

    Department of Molecular Biosciences

    Northwestern University

     

    Protein Biochemistry

    1. Protein Expression and Purification

      1. Bacterial overexpression

      2. FPLC guidelines

      3. DEAE guidelines

      4. In-Vitro Translation

        1. Invitro Translation

        2. Nuclease Treatment of Rabbit Lysate

        3. Coupled translation/transcription

      5. Proteins

        1. Hsp70 columns

        2. Brian Freeman’s hsp70

        3. Jaewan Song’s hsp70

        4. Hip

        5. Hdj-1

        6. GST-Bag-1mm

        7. C. elegans CHIP

    2. Protein Analysis

      1.  Electrophoresis

        1. SDS-PAGE (denaturing)

        2. Native SDS-PAGE/SOD active stain using native gel

        3. 2-dimensional

        4. Blatter gel

      2.  Antibodies

        1. Table of antibodies

        2. Western blot analysis using ECL

        3. Semi-Dry Transfer

        4. Western Blot Analysis Using Alkaline Phosphatase

        5. Western Blot Using Licor Odyssey Analysis

        6. Immunoprecipation

          1. general

          2. HSF1 and HSF2

        7. Immunohistochemistry

      3. Bio-rad assay for quantification

    3. DNA-Protein Interactions

      1. Nuclear extracts

      2. Whole cell extracts

      3. Gel mobility shift analysis

      4. Super Shift

      5. 32P labeling of oligonucleotide probe

      6. In-vitro footprinting methods

        1. Genomic DNA isolation

        2. Ligation mediated PCR

      7. ChIP

    4. Protein-Protein Interactions

      1. Glutaraldehyde and EGS crosslinking

      2. Equilibrium micro-dialysis

      3. GST-pulldown

    5. Filter Trap Assay for Aggregates

    6. Proteomics

      1. Global quantitative proteomics

    7. Re-Folding Assays

      1. Brian Freeman’s ATPase

      2. b-galactosidase

      3. In-vivo refolding

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